Journal: Scientific Reports
Article Title: The role of titanium surface micromorphology in MG-63 cell motility during osteogenesis
doi: 10.1038/s41598-022-13854-2
Figure Lengend Snippet: The variation of implant surface morphology affected the cell movement velocity (CMV). ( a ) Design of the tissue-on-chip (TOC). ( b ) The instrument connections of the microfluidic pressure and flow control system. ( c ) 3D reconstruction of the TOC; the velocity contour of the TOC-T and TOC-C, generated using Fluent 19.0. The red arrows indicate that CMV increases when the fluid passes through the smaller cross-sectional area of the bone cavity. ( d ) Live images and velocity vector diagrams of MG-63 cells traversing the TOC-T and TOC-C. ( e ) The CMV of group T was significantly lower than that of group C ( P < 0.05); there was no significant difference in the CMA of groups T and C ( P > 0.05). ( f ) The values of Sv, contact angle, and zeta potential of groups T and C; Factors contributing to high CMV: Sa and Ssk negatively influenced the high CMV of MG-63, accounting for − 32% and − 51%, respectively ( P < 0.05). Sv , contact angle, and zeta potential did not affect CMV ( P > 0.05); the hydrogen content was an indirectly positive factor for high CMV ( P < 0.05). The statistical significance was set at P < 0.05. This figure was generated by Adobe photoshop CC 2015.
Article Snippet: The ELVEFLOW microfluidic pressure and flow control system included a pressure controller (Fluidic Lab), a flow sensor (Fluidic Lab), and the software ELVEFLOW Smart Interface (ESI) chose the pressure channel: OB1 MK3 + (ELVEFLOW, France).
Techniques: Control, Generated, Plasmid Preparation, Zeta Potential Analyzer